Indian Journal of Experimental Biology
In Drosophila developmental biological studies, X-gal staining is commonly employed to study the spatio-temporal expression of the lacZ reporter gene in the transformed flies or their embryos. Study of the lacZ pattern in embryos often suffers from the lack of an efficient and high yieldirrg technique for devitellinization of X-gal stained embryos. Devitellinization techniques employed during antibody staining, in situ hybridization or embryonic cuticular preparations generally do not give satisfactory results when used for similar purpose in X-gal stained embryos. This results in the flaky appearance of the blue stain. We present here an improved chemical devitellinization technique which gives a high yield of devitellinized embryos and a better resolution of the X-gal staining pattern. ...
We present here a chemical devitellinization technique for X-gal stained embryos where the major hurdles of time consumption, low yield of devitellinized embryos, chances of loss of morphology of the embryos or quality of stain have been overcome.
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Singh, Amit; Kango-Singh, Madhuri; and Sinha, P., "An Improved Method for Chemical Devitellinization of X-gal Stained Drosophila Embryos" (1995). Biology Faculty Publications. 123.