Excretion and Conservation of Glycerol, and Expression of Aquaporins and Glyceroporins, During Cold Acclimation in Cope's Gray Tree Frog Hyla Chrysoscelis
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology
Cope's gray tree frog Hyla chrysoscelis accumulates glycerol during cold acclimation. We hypothesized that, during this process, gray tree frogs adjust renal filtration and/or reabsorption rates to retain accumulated glycerol. During cold acclimation, plasma concentrations of glycerol rose >200-fold, to 51 mmol/l. Although fractional water reabsorption decreased, glomerular filtration rate (GFR) and, consequently, urine flow wereXenopus oocyte expression system. HC-1, an AQP1-like water channel conferring low glycerol permeability, is expressed ubiquitously in warm- and cold-acclimated tissues. HC-2, a water channel most similar to AQP2, is primarily expressed in organs of osmoregulation. HC-3, which is most similar to AQP3, is functionally characterized as a GLP, with low permeability to water but high permeability to glycerol. Aspects of expression levels and functional characteristics varied between cold and warm conditions for each of the three AQPs, suggesting a complex pattern of involvement in osmoregulation related to thermal acclimation.
Copyright © 2007, American Physiological Society
American Physiological Society
National Science Foundation
Zimmerman, Sarah L.; Frisbie, James; Goldstein, David L.; West, Jennifer; Rivera, Kevin; and Krane, Carissa M., "Excretion and Conservation of Glycerol, and Expression of Aquaporins and Glyceroporins, During Cold Acclimation in Cope's Gray Tree Frog Hyla Chrysoscelis" (2007). Biology Faculty Publications. 135.