Title
LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP
Document Type
Article
Publication Date
3-2014
Publication Source
Methods in Molecular Biology
Abstract
The secondary messenger molecule, 3′,5′-cyclic diguanosine monophosphate (c-di-GMP), controls various cellular processes in bacteria. Direct measurement of intracellular concentration of c-di-GMP is fast becoming an important tool for studying prokaryotic biology. Here, we describe a comprehensive extraction protocol from live bacteria and quantitative analysis using LC/MS/MS.
Inclusive pages
271-279
ISBN/ISSN
1064-3745
Copyright
Copyright © 2014, Springer
Publisher
Springer
Volume
1149
Peer Reviewed
yes
eCommons Citation
Irie, Yasuhiko and Parsek, Matthew R., "LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP" (2014). Biology Faculty Publications. 211.
https://ecommons.udayton.edu/bio_fac_pub/211
COinS
Comments
Permission documentation is on file.