Title

LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP

Document Type

Article

Publication Date

3-2014

Publication Source

Methods in Molecular Biology

Abstract

The secondary messenger molecule, 3′,5′-cyclic diguanosine monophosphate (c-di-GMP), controls various cellular processes in bacteria. Direct measurement of intracellular concentration of c-di-GMP is fast becoming an important tool for studying prokaryotic biology. Here, we describe a comprehensive extraction protocol from live bacteria and quantitative analysis using LC/MS/MS.

Inclusive pages

271-279

ISBN/ISSN

1064-3745

Comments

Permission documentation is on file.

Publisher

Springer

Volume

1149

Peer Reviewed

yes