Title

Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in 'Pseudomonas aeruginosa'

Document Type

Book Chapter

Publication Date

2017

Publication Source

c-di-GMP Signaling: Methods and Protocols

Abstract

3′,5′-cyclic diguanosine monophosphate (cyclic di-GMP) is a bacterial secondary messenger molecule that regulates many important cellular activities and behaviors, such as motility and biofilm formation. While mass spectrometry protocols for quantitative analyses of intracellular cyclic di-GMP concentrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a Pseudomonas aeruginosa-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.

ISBN/ISSN

978-1-4936-7239-5

Publisher

Springer

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