Document Type
Editorial
Publication Date
2-10-2022
Publication Source
Antibiotics
Abstract
The application of molecular detection methods for bacterial pathogens has dramatically improved the outcomes of septic patients, including those with methicillin-resistant Staphylococcus aureus (MRSA) infections. Molecular methods can be applied to a variety of clinical specimens including nasal swabs, growth in blood culture bottles, and wounds. While data show that the overall accuracy of molecular tests for MRSA is high, results can be confounded by the presence of multiple staphylococcal species in a specimen, insertions and deletions of DNA in and around the Staphylococcal Cassette Chromosome mec (SCCmec) element, and point mutations in mecA. Herein, we explore the complexities of molecular approaches to MRSA detection and the instances where phenotypic methods should be pursued to resolve discrepancies between genotypic and phenotypic results.
ISBN/ISSN
ISSN: 2079-6382
Document Version
Published Version
Publisher
MDPI
Volume
11
Issue
2
Keywords
MRSA, MSSA, PCR, mecA, blood culture, methicillin resistance
eCommons Citation
Tenover, Fred C. and Tickler, Isabella A., "Detection of Methicillin-Resistant Staphylococcus aureus Infections Using Molecular Methods" (2022). Biology Faculty Publications. 355.
https://ecommons.udayton.edu/bio_fac_pub/355
Included in
Biology Commons, Biotechnology Commons, Cell Biology Commons, Genetics Commons, Microbiology Commons, Molecular Genetics Commons
Comments
This document is made available for download in compliance with the publisher’s open-access policy. Permission documentation is on file.