Matthew E. Lopper
Deinococcus radiodurans is a species of bacteria that has sparked a lot of interest since its discovery due to its incredible resistance to ionizing radiation. When exposed to ionizing radiation the genome of D. radiodurans will sustain over one hundred double stranded breaks. D. radiodurans demonstrates the ability to repair its genome and restart replication after sustaining, typically lethal, DNA damage. This project examined the mechanism of replication restart in D. radiodurans by investigating primosome protein PriA interacting with replicative helicase DnaB and single stranded binding protein. Many different types of gel electrophoresis were employed to investigate potential protein complex formations between D. radiodurans PriA and DnaB. Native agarose gel electrophoresis successfully revealed an interaction between D. radiodurans PriA and DnaB. Results in this work indicate that D. radiodurans PriA can interact with DnaB.
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Chemistry | Physical Sciences and Mathematics
Morrow, Christopher S., "Exploration of Protein-Protein Interactions Involving Deinococcus radiodurans PriA, DnaB and SSB" (2015). Honors Theses. 64.