Different Methods for Culturing Biofilms In Vitro

Author(s)

S. Brook Peterson, University of Washington
Yasuhiko Irie, University of DaytonFollow
Bradley R. Borlee, Colorado State University
Keiji Murakami, University of Washington - Seattle Campus
Joe J. Harrison, University of CalgaryFollow
Kelly M. Colvin, University of Washington - Seattle Campus
Matthew R. Parsek, University of Washington - Seattle Campus

Document Type

Book Chapter

Publication Date

10-2010

Publication Source

Biofilm Infections

Abstract

The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved. One of the underlying reasons is the lack of standardized methods for culturing biofilm communities. Many times, the culturing format will be unique to the study in question, resulting in difficulties when other labs attempt to confirm results produced by another lab. Another issue has been the limited utility of different culturing methods for the specific research questions being asked. For example, culturing formats designed to be accessible to microscopy are not always suited for other types of analyses, such as harvesting biofilm biomass for biochemical measurements.

Inclusive pages

251-266

ISBN/ISSN

978-1-4419-6083-2

Comments

Permission documentation is on file.

Copyright

Copyright © 2010, Springer

Publisher

Springer

Place of Publication

New York, NY

Peer Reviewed

yes

eCommons Citation

Peterson, S. Brook; Irie, Yasuhiko; Borlee, Bradley R.; Murakami, Keiji; Harrison, Joe J.; Colvin, Kelly M.; and Parsek, Matthew R., "Different Methods for Culturing Biofilms In Vitro" (2010). Biology Faculty Publications. 204.
https://ecommons.udayton.edu/bio_fac_pub/204