Journal of Clinical Microbiology
Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culture-positive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample was
Print: 0095-1137; Electronic: 1098-660X
American Society for Microbiology
Streptococcus agalactiae, group B streptococcus, whole-genome sequencing
Tickler, Isabella A.; Tenover, Fred C.; Dewell, Scott; Le, Victoria M.; Blackman, Rachel N.; Goering, Richard V.; Rogers, Amy E.; Piwonka, Heather; Jung-Hynes, Brittney D.; Chen, Derrick J.; Loeffelholz, Michael J.; Gnanashanmugam, Devasena; and Baron, Ellen Jo, "Streptococcus agalactiae Strains with Chromosomal Deletions Evade Detection with Molecular Methods" (2019). Biology Faculty Publications. 343.