Title
Streptococcus agalactiae Strains with Chromosomal Deletions Evade Detection with Molecular Methods
Document Type
Article
Publication Date
4-2019
Publication Source
Journal of Clinical Microbiology
Abstract
Surveillance of circulating microbial populations is critical for monitoring the performance of a molecular diagnostic test. In this study, we characterized 31 isolates of Streptococcus agalactiae (group B Streptococcus [GBS]) from several geographic locations in the United States and Ireland that contain deletions in or adjacent to the region of the chromosome that encodes the hemolysin gene cfb, the region targeted by the Xpert GBS and GBS LB assays. PCR-negative, culture-positive isolates were recognized during verification studies of the Xpert GBS assay in 12 laboratories between 2012 and 2018. Whole-genome sequencing of 15 GBS isolates from 11 laboratories revealed four unique deletions of chromosomal DNA ranging from 181 bp to 49 kb. Prospective surveillance studies demonstrated that the prevalence of GBS isolates containing deletions in the convenience sample was
ISBN/ISSN
Print: 0095-1137; Electronic: 1098-660X
Document Version
Postprint
Publisher
American Society for Microbiology
Volume
57
Peer Reviewed
yes
Issue
4
Keywords
Streptococcus agalactiae, group B streptococcus, whole-genome sequencing
eCommons Citation
Tickler, Isabella A.; Tenover, Fred C.; Dewell, Scott; Le, Victoria M.; Blackman, Rachel N.; Goering, Richard V.; Rogers, Amy E.; Piwonka, Heather; Jung-Hynes, Brittney D.; Chen, Derrick J.; Loeffelholz, Michael J.; Gnanashanmugam, Devasena; and Baron, Ellen Jo, "Streptococcus agalactiae Strains with Chromosomal Deletions Evade Detection with Molecular Methods" (2019). Biology Faculty Publications. 343.
https://ecommons.udayton.edu/bio_fac_pub/343
Included in
Biology Commons, Biotechnology Commons, Cell Biology Commons, Genetics Commons, Microbiology Commons, Molecular Genetics Commons
Comments
The document available for download is the authors' accepted manuscript, provided to PubMed Central with the permission of the publisher. Permission documentation is on file.
PMCID: PMC6440789
PMID: 30760532
To view the version of record, use the DOI: https://doi.org/10.1128/jcm.02040-18