Characterization of Carbapenem-Resistant Gram-Negative Bacterial Isolates from Nigeria by Whole Genome Sequencing
Diagnostic Microbiology and Infectious Disease
This study characterized the mechanisms of carbapenem resistance in gram-negative bacteria isolated from patients in Yola, Nigeria. Whole genome sequencing (WGS) was performed on 66 isolates previously identified phenotypically as carbapenem-non–susceptible.
The patterns of beta-lactamase resistance genes identified were primarily species-specific. However, blaNDM-7 and blaCMY-4 were detected in all Escherichia coli and most Providencia rettgeri isolates; blaNDM-7 was also detected in 1 Enterobacter cloacae. The E. coli and E. cloacae isolates also shared blaOXA-1, while blaOXA-10 was found in all P. rettgeri, one Pseudomonas aeruginosa and 1 E. coli. Except for Stenotrophomonas maltophilia isolates, which only contained blaL1, most species carried multiple beta-lactamase genes, including those encoding extended-spectrum beta-lactamases, AmpC and OXA in addition to a carbapenemase gene. Carbapenemase genes were either class B or class D beta-lactamases. No carbapenemase gene was detected by WGS in 13.6% of isolates.
Print ISSN: 0732-8893; Online ISSN: 1879-0070
Carbapenem resistance, Carbapenemase, Beta-lactamase
Tickler, Isabella A. BS; Tenover, Fred C. PhD; dela Cruz, Caitlin M. BS; Shettima, Shuwaram A. MBBS; Le, Victoria M. BS; Dewell, Scott PhD; and Sumner, Jack BS, "Characterization of Carbapenem-Resistant Gram-Negative Bacterial Isolates from Nigeria by Whole Genome Sequencing" (2021). Biology Faculty Publications. 360.