Fetuin-A Adsorption, Stabilization, and Solubilization of Amorphous Calcium Carbonate Nanoparticles

Document Type

Article

Publication Date

7-7-2015

Publication Source

Journal of Materials Chemistry B

Abstract

Fetuin-A is a serum glycoprotein identified as a calcification inhibitor, and a key player in bone formation and human metabolic processes. A study on binding mechanisms of Fetuin-A with calcium carbonate nanoparticles in a simulated body fluid (DMEM) environment is presented. Observed interactions between Fetuin-A and the CaCO3 nanoparticles reveal an initial adsorption process, followed by a stabilization stage, and then a solubilization period for the Fetuin-A/CaCO3 complex. FTIR and XPS are used to monitor functional group and elemental composition changes during the initial adsorption process between Fetuin-A and the CaCO3 nanoparticles. Distinctive Fetuin-A/CaCO3 complex structures—also known as mineralo-protein particles—are imaged with TEM and SEM. DLS and UV-Vis methods are used to further characterize the in situ binding mechanisms. Results of this study can guide the design of complex organic–inorganic hybrid materials, improve current drug delivery methods, and provide insight in monitoring and controlling interactions between Fetuin-A and external calcium ions.

Inclusive pages

6411-6419

ISBN/ISSN

2050-7518

Document Version

Postprint

Comments

The document available for download is the authors' accepted manuscript, provided in compliance with the publisher's policy on self-archiving. Permission documentation is on file. To view the version of record, use the DOI: https://doi.org/10.1039/C5TB00565E

Publisher

Royal Society of Chemistry

Volume

3

Peer Reviewed

yes

Issue

31

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