Title

Development of a Fluorescence Polarization Assay for Influenza Polymerase Acidic Protein Inhibitors

Date of Award

8-8-2022

Degree Name

M.S. in Chemistry

Department

Department of Chemistry

Advisor/Chair

Doug Daniels

Abstract

Influenza is a global health threat. It has been estimated by the World Health Organization that influenza is responsible for approximately 290,000-650,000 deaths, annually[1]. While there are vaccines and antiviral drugs available, there is a limit on the effectiveness of these methods of combatting the threat of influenza. Vaccines require annual updates and most of the current antivirals are experiencing an explosion of drug resistance. To improve upon these limitations, influenza's polymerase acidic (PA) protein offers much potential as a new antiviral drug target. PA is highly conserved among various strains of influenza and plays a vital role in viral replication. Therefore, an antiviral drug that successfully targets PA will be a viable therapeutic option for a longer duration, as compared to drugs that target viral proteins with a higher amino acid variability. This thesis will showcase the process of the development of a fluorescence polarization assay to validate potential PA inhibitors for further antiviral advancements.

Keywords

Biohemistry, Department of Chemistry, fluorescence polarization, assay development, polymerase acidic protein, influenza

Rights Statement

Copyright © 2022, author.

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