Using RNA-interference to Identify the Genetic Toolkit for a Fruit Fly Morphological Trait
Metazoan animal morphological traits result from the combined inputs of hundreds or more genes that comprise gene regulatory networks (GRNs). Each GRN utilizes various genes from the genetic toolkit of transcription factor and signaling pathway genes to control complex patterns of gene expression. However, for few traits, if any, has the full repertoire of toolkit genes been characterized for its GRN. Thus, how traits are built by regulated gene expression remains poorly understood. For my thesis, I am investigating the genetic toolkit that makes a male-specific pigmentation for the fruit fly species Drosophila melanogaster. Using RNA-interference, I will reduce the expression of ~190 transcription factor and ~21 signaling pathway genes to find those necessary for this trait by surveying for deviant pigmentation phenotypes. The results will provide a greater understanding of how a GRN is able to direct the formation of a well formed trait.
Tom M Williams
Primary Advisor's Department
Stander Symposium poster
"Using RNA-interference to Identify the Genetic Toolkit for a Fruit Fly Morphological Trait" (2018). Stander Symposium Posters. 1124.