Role of M1BP in eye development of Drosophila melanogaster


Role of M1BP in eye development of Drosophila melanogaster



Hannah Corinne Gordon, Abijeet Singh Mehta, Ankita Sarkar, Kaitlyn E Strickland



Regulation of transcription in multi-cellular organisms is responsible for generating diversity in cell type and final patterning and growth of an organ. The regulation of gene expression can be present at multiple levels like assembling pre-initiation complex at promoters or regulation of gene expression by micro RNA. Recently, a novel transcription factor M1BP (TF; Motif 1 binding protein) has been identified which is required to regulate a large class of paused genes. However, the role of the Motif 1 binding protein is unknown in the eye development. We employed Drosophila melanogaster eye model to understand its role in patterning, growth and development. M1BP is highly conserved across the species and encodes a 55kDa protein containing five C2H2 zinc-fingers domains. A battery of highly conserved genes regulates Drosophila eye development. In our studies, we examine the effects of Motif 1 Binding Protein (M1BP), and its effects on the control of gene expression during eye development of the Drosophila melanogaster. Our preliminary data suggests that absence of M1BP function in dorsal and ventral eye margins results in the suppression of eye fate and the suppression of the gene from the entire eye gives us a head loss phenotype. We also hypothesize that the loss of function o f M1BP leads to the change of the fate of peripodial epithelium cells to disc proper in the eye imaginal disc. The above data will be presented in our poster.

Publication Date


Project Designation

Honors Thesis

Primary Advisor

Madhuri Kango-Singh, Amit Singh

Primary Advisor's Department



Stander Symposium project

Role of M1BP in eye development of Drosophila melanogaster