Methods of Blocking Efflux Pump Activity in Escherichia Coli
Erich Nicholaus Auer
Overuse of antibiotics throughout the developed world has contributed significantly to the emergence of antibiotic-resistance in bacteria. Although this helps bacteria it is extremely harmful to humans. As bacteria adapt to the antibiotics, current treatments become ineffective and bacterial infections can become life threatening. One of the mechanisms that bacteria, such as E. Coli, use are efflux pumps. Efflux pumps are capable of taking potentially harmful substances, like antibiotics, from inside the bacterial cell and moving them out. This allows the bacterium to escape from the killing effects of the antibiotics. My hypothesis was that a natural product, small molecule compound, or DNA aptamer would be able to bind to some part of the tri-part structure of the efflux pump and block its activity. The results showed that there are some promising compounds but none that were actually capable of blocking activity to a significant degree.
Matthew E. Lopper
Primary Advisor's Department
Stander Symposium project
"Methods of Blocking Efflux Pump Activity in Escherichia Coli" (2018). Stander Symposium Projects. 1389.