Antibiotic Producing Bacteria in Soil Samples at the University of Dayton
Erin Elise Pellot
Within recent years there has been a rise in the number of antibiotic resistant bacterial strains. These resistant strains have no known treatments and thus pose a threat to society. Bacterial strains form resistance by evolving past the mechanisms behind a given antibiotic. With bacteria’s unrelenting ability to evolve and adapt there is an ever present need for new antibiotics. To relieve this need, the Tiny World Initiative was formed to provide students the chance to conduct antibiotic research in the classroom setting. For this in class research experience, an on-campus soil sample was collected and analyzed for possible antibiotic producing microbes. After cultivating and selecting bacterial colonies, samples were tested against two pathogen safe relatives of the ESKAPE pathogens. ESKAPE is an acronym to describe the six most antibiotic resistant opportunistic pathogens which pose a threat to society. The ESKAPE relatives tested against in this experiment were Pseudomonas putida and Staphylococcus epidermidis. After finding seven antibiotic producing colonies the samples were put through multiple classification tests which include a gram stain, catalase test, hemolysis, triple sugar iron, mannitol salt agar, MacConky agar, citrate, gelatinase and a motility test. After classifying the samples they were tested against Eukaryotic cells in the hopes of finding a safe antibiotic treatment.
Erica Marie Rinehart, Yvonne Y. Sun
Primary Advisor's Department
Stander Symposium project
"Antibiotic Producing Bacteria in Soil Samples at the University of Dayton" (2019). Stander Symposium Projects. 1517.