Investigating the Potential Antimicrobial Effects of Soil Isolates from UD’s Campus
Kaylin Ann Kultgen
Throughout this past semester, I have been doing research in BIO 411L (Microbiology Lab) using procedures established by the Small World Initiative. The goal of the research is to isolate bacteria that displays anti-microbial effects on known pathogens. This research could potentially be used to help create new antibiotics to fight against human pathogens. A dirt sample was collected from the side of my house in the UD south student neighborhood. Colonies of bacteria formed on TH and TSA plates. Ten colonies were chosen from each of these types of agar plates. The colonies were plated on dishes streaked with the known pathogens, Pseudomonas aeruginosa and Enterococcus faecium, then later Klebsiella pneumoniae, and Staphylococcus aureus. The colonies that formed zones of inhibition were isolated and further testing was done to determine the species and characteristics of the bacteria. Some of these tests included a gram stain, catalase test, and various other biochemical tests to determine characteristics of the antibiotic producing bacteria. Later this semester, I will perform a chemical extraction for the metabolites of the chosen bacteria as well as observe the interaction between the extract and eukaryotic cells.
Erica Marie Rinehart, Yvonne Y. Sun
Primary Advisor's Department
Stander Symposium project
"Investigating the Potential Antimicrobial Effects of Soil Isolates from UD’s Campus" (2019). Stander Symposium Projects. 1527.