Neurochemical alterations upon retinectomy in the developing chick embryo: a preliminary study

Title

Neurochemical alterations upon retinectomy in the developing chick embryo: a preliminary study

Authors

Presenter(s)

John Richard Coffey

Files

Description

Chick embryos, between 3.5 and 4.5 days of development, have been found to be able to completely regenerate a removed retina in the presence of fibroblast growth factor 2 (FGF2). FGF2 activates the trans-differentiation of the retinal pigmented epithelium, providing a necessary step in the regenerative process. While the regenerative capabilities of the chick embryo are known, the injury signals, which stem from the retinal injury, effect on the brain’s neurochemistry is not known. The ISE summer CoRPs project aimed to use HPLC analysis of the chick embryo brain tissue during periods of 30 minutes, two hours, and three days post-retinectomy for both embryos with and without fibroblast growth factor 2 to analyze the impact injury signals and subsequent regeneration had on the brain’s neurochemistry. Samples of embryo brain tissue were harvested at Miami University and brought back to the University of Dayton, where they were subjected to ex vivo neurochemical analysis with high performance liquid chromatography. Herein, we present first preliminary evidence for intriguing neurochemical alterations upon retinectomy in the chick embryo.

Publication Date

4-22-2020

Project Designation

Independent Research

Primary Advisor

Pothitos Pitychoutis

Primary Advisor's Department

Biology

Keywords

Stander Symposium Posters, College of Arts and Sciences

United Nations Sustainable Development Goals

Good Health and Well-Being

Neurochemical alterations upon retinectomy in the developing chick embryo: a preliminary study

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