Neurochemical alterations upon retinectomy in the developing chick embryo: a preliminary study
John Richard Coffey
Chick embryos, between 3.5 and 4.5 days of development, have been found to be able to completely regenerate a removed retina in the presence of fibroblast growth factor 2 (FGF2). FGF2 activates the trans-differentiation of the retinal pigmented epithelium, providing a necessary step in the regenerative process. While the regenerative capabilities of the chick embryo are known, the injury signals, which stem from the retinal injury, effect on the brain’s neurochemistry is not known. The ISE summer CoRPs project aimed to use HPLC analysis of the chick embryo brain tissue during periods of 30 minutes, two hours, and three days post-retinectomy for both embryos with and without fibroblast growth factor 2 to analyze the impact injury signals and subsequent regeneration had on the brain’s neurochemistry. Samples of embryo brain tissue were harvested at Miami University and brought back to the University of Dayton, where they were subjected to ex vivo neurochemical analysis with high performance liquid chromatography. Herein, we present first preliminary evidence for intriguing neurochemical alterations upon retinectomy in the chick embryo.
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
"Neurochemical alterations upon retinectomy in the developing chick embryo: a preliminary study" (2020). Stander Symposium Projects. 1782.