Genetic interaction between the axial patterning gene defective proventriculus (dve) and Dpp signaling in the developing Drosophila eye
Anuradha Chimata Venkatakrishnan, Neha Gogia, Katie Marie Perry
An interesting question in developmental biology is how any three-dimensional organ develops from a single monolayer of cells. In multicellular organisms, delineation of Antero-Posterior (AP), Dorso-Ventral (DV) and Proximo-Distal (PD) axes is crucial for organ development. Drosophila melanogaster is an ideal model organism because genes and pathways are highly conserved between Drosophila and humans. During eye development, DV axis formation marks the first lineage restriction event and deviation in this process results in birth defects in the eye. We have identified defective proventriculus (dve) as a new dorsal eye fate selector in Drosophila. We hypothesize that dve, a transcription factor, regulates the Decapentaplegic (Dpp) signaling pathway, which is comparable to bone morphogenetic protein (BMP) signaling in mammals, during dorsal eye development. We will investigate the role of dve in the dorsal eye and whether Dpp signaling requires dve to specify eye and head fate to form an organ of balanced size and shape. We utilized the GAL4-UAS system to modulate Dpp signaling in the dve domain. Thus far in our research, we have found that upregulating Dpp in the dve domain results in an enlarged eye phenotype, while downregulating Dpp in the dve domain results in a small eye phenotype. We have tested retinal determination genes to explore cell fate and further support the genetic interaction between dve and Dpp signaling. We are currently testing their interaction using clonal strategies to understand how dve regulates dpp in the developing eye. This study may have a significant bearing on growth, signaling and patterning defects and help in understanding the etiology behind genetic birth defects in the eye.
Madhuri Kango-Singh, Amit Singh
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
"Genetic interaction between the axial patterning gene defective proventriculus (dve) and Dpp signaling in the developing Drosophila eye" (2020). Stander Symposium Projects. 2006.