Structure-function analysis of Defective proventriculus (Dve) in Drosophila melanogaster eye growth and development
Anuradha Chimata Venkatakrishnan
During development, axial patterning is required to establish Antero-posterior (AP), Dorso-Ventral (DV), and Proximo-Distal (PD) axes, which is crucial for the generation of a 3-dimensional organ from a monolayer organ primordium. Of the three axes, DV axis is the first lineage restriction event during eye development and any deviation results in developmental birth defects. In our study, we have used Drosophila melanogaster (Fruit fly) eye as a model system to understand the role of different domains of a new dorsal eye fate selector gene, defective proventriculus (dve, an ortholog of SATB1) in growth and development. In humans, SATB1, functions as a transcriptional regulator and chromatin organizer and requires tetramerization by the ULD domain. In Drosophila eye, dve regulates expression of wingless (wg), a negative regulator of eye. In the genetic hierarchy, dve acts downstream of GATA-1 transcription factor pannier (pnr) and upstream of wg. Loss-of-function of dve results in dorsal eye enlargement while gain-of-function results in eye suppression. We performed structure function analysis of Dve protein to elucidate the role of various domains in patterning, growth and development. We have developed several transgenic lines, which will allow us to induce expression of the specific domains of Dve protein and assay their effect on Drosophila eye growth and development. Dve has a ULD domain for tetramerization, HOX domains for DNA binding and PPP4R2 domain for H2AFX dephosphorylation. Here we present our results on ectopic induction of these domains and their effect on eye phenotype and wg expression in the developing eye.
Madhuri Kango-Singh, Amit Singh
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
"Structure-function analysis of Defective proventriculus (Dve) in Drosophila melanogaster eye growth and development" (2021). Stander Symposium Projects. 2099.