John Paul A. Yoseph



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Given that antibiotics are being used worldwide to treat various bacterial infections and diseases, antibiotic resistance has become an increasingly mainstream and widespread issue; therefore, causing many antibiotics to lose effectiveness over time in treatment. As a result, research in the field of bacterial resistance to antibiotics has become increasingly popular and widely demanded as we search to produce new effective antibiotics. Bacteria produce these antimicrobials when put in an environment with present pathogens or with limited resources, causing either a competition for survival or a need to fight infection. These antibiotics can either be created synthetically, or can be removed and isolated from bacterial colonies with antimicrobial properties. This independent research aims to observe isolates of bacteria from specific soil samples, while deciding if the isolates display any antimicrobial properties in an environment with antibiotic resistant pathogens. Zone of inhibitions will be generated, indicating antimicrobial properties in the existence of Bacillus subtilis, Erwinia carotovora, Escherichia coli, and Staphylococcus epidermis. Bacteria which generate antimicrobial properties will be inspected additionally by a sequence of biochemical tests, gram staining and catalase testing. In establishing and recognizing which bacteria produce antimicrobial agents and demonstrate these properties, these procedures will be crucial to fight the rise of antibiotic resistance, and to create effective new antibiotics.

Publication Date


Project Designation

Independent Research

Primary Advisor

Jessica Elizabeth Geyer, Yvonne Y. Sun

Primary Advisor's Department



Stander Symposium project, College of Arts and Sciences

United Nations Sustainable Development Goals

Good Health and Well-Being; Life On Land

Antibiotic Discovery Research Using Soil Samples: Microbiology Undergraduate Research