Anuradha Chimata Venkatakrishnan, Imaan Jameel Khan
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All multicellular organisms show strictly controlled transcriptional regulation that determines differential gene expression along the spatiotemporal axis. This dynamic spatiotemporal gene expression determines the variation in cellular structure and functions and also their interaction with other genes or proteins in cells. So far, GFP or LacZ based reporter systems have been widely utilized in Drosophila melanogaster to study such gene expression changes. Alternatively, antibody based immunohistochemical approach is also used to determine protein localization patter in tissues. But such approaches pose a challenge when studying transient or very early expression patterns. Therefore, we have utilized a genetic system that combines Gal4/UAS, FLP/FRT and fluorescent reporters to provide information about spatial, temporal, and lineage expression of genes. When crossed with a Gal4 stock, the Gal4 Technique for Real-time and Clonal Expression (G-TRACE) will reveal real time RFP expression, and ubiquitous lineage-traced GFP expression. Here, we utilize this method to study some of the genes that play an important role during eye development.
Madhuri Kango-Singh, Amit Singh
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
"Studying spatiotemporal gene expression in real time and lineage cells in Drosophila using G-Trace reporter system" (2022). Stander Symposium Projects. 2426.