Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) - non-structural protein 3 suppresses the pro-inflammatory cytokines in host cell
Ryan Jamison Nelson
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, single-stranded positive-sense RNA virus with genome size ~30 Kb. SARS-CoV-2 genome encodes 13–15 open reading frames (ORFs). Among them ORF1a encodes a non-structural protein 3 (Nsp3). Nsp3 is one of the largest proteins encoded by virus with an average molecular mass of about 200 kD. Nsp3 plays an important role in virus replication which includes creating virus replication/transcription complex and processing viral proteins. Nsp3 with papain-like protease activity cleave Nsp1-Nsp2, Nsp2-Nsp3 and Nsp3-Nsp4 in viral polypeptide. However, there is very little known about the role of Nsp3 on host innate immune response. To determine the role of SARS-Cov2- Nsp3 on host innate immune response, we transfected the HEK-293 Cell with plasmid containing SARS-CoV2-Nsp3 gene with green fluorescent protein (GFP). Transfected cells were analyzed for interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF alpha) expression. Our result showed that SARS-Cov2- Nsp3 significantly reduced IL-6 and TNF alpha expression, indicating the role of SARS-Cov2- Nsp3 in virus induced immunosuppression. However, we need to confirm our results with more replicates
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being; Clean Water and Sanitation
"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) - non-structural protein 3 suppresses the pro-inflammatory cytokines in host cell" (2022). Stander Symposium Projects. 2434.