Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) - non-structural protein 3 suppresses the pro-inflammatory cytokines in host cell

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) - non-structural protein 3 suppresses the pro-inflammatory cytokines in host cell

Presenter(s)

Ryan Jamison Nelson

Comments

Presentation: 9:00 a.m.-10:15 a.m., Kennedy Union Ballroom

Description

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, single-stranded positive-sense RNA virus with genome size ~30 Kb. SARS-CoV-2 genome encodes 13–15 open reading frames (ORFs). Among them ORF1a encodes a non-structural protein 3 (Nsp3). Nsp3 is one of the largest proteins encoded by virus with an average molecular mass of about 200 kD. Nsp3 plays an important role in virus replication which includes creating virus replication/transcription complex and processing viral proteins. Nsp3 with papain-like protease activity cleave Nsp1-Nsp2, Nsp2-Nsp3 and Nsp3-Nsp4 in viral polypeptide. However, there is very little known about the role of Nsp3 on host innate immune response. To determine the role of SARS-Cov2- Nsp3 on host innate immune response, we transfected the HEK-293 Cell with plasmid containing SARS-CoV2-Nsp3 gene with green fluorescent protein (GFP). Transfected cells were analyzed for interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF alpha) expression. Our result showed that SARS-Cov2- Nsp3 significantly reduced IL-6 and TNF alpha expression, indicating the role of SARS-Cov2- Nsp3 in virus induced immunosuppression. However, we need to confirm our results with more replicates

Publication Date

4-20-2022

Project Designation

Capstone Project

Primary Advisor

Mrigendra Rajput

Primary Advisor's Department

Biology

Keywords

Stander Symposium project, College of Arts and Sciences

United Nations Sustainable Development Goals

Good Health and Well-Being; Clean Water and Sanitation

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) - non-structural protein 3 suppresses the pro-inflammatory cytokines in host cell

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