ZFP36 Ring Finger Protein Like 1 (ZFP36L1) knockdown significantly reduced lipopolysaccharide-induced proinflammatory cytokine expression
Tooba Shafeeque Ahmed Momin, Andrew Villasenor
CCCH-Type Zinc finger proteins(CCCH-ZFP) are small protein domains that are structurally maintained by zinc ions. Zinc ions coordinate the protein structure in a tetrahedral geometry by biding cysteines or cysteines and histidine amino acids. The unique structure of CCCH-ZFP enables it to interact with a wide variety of molecules such as DNA, RNA, or cellular proteins and thus modulate several cellular processes including host immune response and virus replication. For the current study, we screened 68 CCCH type zinc finger proteins using a literature search for their antiviral as well as immunomodulatory properties along with their expression in human cells and their potential to interact with SARS-CoV-2 RNA using RNA-Protein Interaction Prediction (RPISeq) software. Using this strategy, we selected ZFP36 Ring Finger Protein Like 1 (ZFP36L1) which scored a higher point to interact with SARS-CoV-2 RNA and modulate host immune response as compared to other CCCH type zinc finger proteins. Before measuring the effect of ZFP36L1 expression on SARS-CoV-2 replication, we aimed to determine the effect of ZFP36L1 expression on host innate immune response. We overexpressed or knockdown ZFP36L1 in HEK 293T cells as well as in Raw 264.7 macrophage. Our preliminary results showed that knocking down ZFP36L1 significantly reduced lipopolysaccharide (LPS) mediated tumor necrosis factor-alpha(TNF alpha) expression (p<0.05). However, we still need to measure the effect of ZFP36L1 overexpression or knockdown on LPS induced TNF alpha at earlier timepoints.
Primary Advisor's Department
Stander Symposium project, College of Arts and Sciences
United Nations Sustainable Development Goals
Good Health and Well-Being
"ZFP36 Ring Finger Protein Like 1 (ZFP36L1) knockdown significantly reduced lipopolysaccharide-induced proinflammatory cytokine expression" (2022). Stander Symposium Projects. 2449.