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This study focuses on the engineering and design of a biconic, tapered optical fiber platform for biosensing applications. The sensor platform consists of a machined polytetrafluoroethylene (Teflon, PTFE) flow cell which is chemically inert, easily machined, and available at low-cost. The flow cell was designed to withstand temperatures of 0 to 60 degrees Celsius, to protect the fragile, tapered fiber, and connected to a syringe pump to allow for the introduction of aqueous solutions for surface chemistry functionalization and analyte exposures. The flow system was used to characterize individual single-mode or polarization-maintaining fibers that were tapered to a waist diameter of approximately 10 microns. Signal was measured as the amount of light transmission through the tapered fibers. Preliminary results obtained for antibody-antigen interactions indicate that molecular interaction of the antigen with the surface-tethered antibody results in a change in the quantity and phase of light passing through the tapered fiber.

Publication Date


Project Designation

Independent Research

Primary Advisor

Karolyn M. Hansen

Primary Advisor's Department



Stander Symposium poster

Fabrication of Low-Cost Flow Cell and Tapered Optical Fibers for Aqueous Biosensing