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This study focuses on the engineering and design of a biconic, tapered optical fiber platform for biosensing applications. The sensor platform consists of a machined polytetrafluoroethylene (Teflon, PTFE) flow cell which is chemically inert, easily machined, and available at low-cost. The flow cell was designed to withstand temperatures of 0 to 60 degrees Celsius, to protect the fragile, tapered fiber, and connected to a syringe pump to allow for the introduction of aqueous solutions for surface chemistry functionalization and analyte exposures. The flow system was used to characterize individual single-mode or polarization-maintaining fibers that were tapered to a waist diameter of approximately 10 microns. Signal was measured as the amount of light transmission through the tapered fibers. Preliminary results obtained for antibody-antigen interactions indicate that molecular interaction of the antigen with the surface-tethered antibody results in a change in the quantity and phase of light passing through the tapered fiber.
Karolyn M. Hansen
Primary Advisor's Department
Stander Symposium poster
Edwards, Marika S.; Grandinette, Dillon T.; King, Branden J.; and Melendez, Jonathan B., "Fabrication of Low-Cost Flow Cell and Tapered Optical Fibers for Aqueous Biosensing" (2013). Stander Symposium Posters. 263.