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Fluidic organ-on-chip (OOC) devices are powerful tools in biomedical research, allowing for the set-up, control, and monitoring of complex biological scenarios that better mimic in vivo conditions. Currently, the adoption of OOC devices for biological research is limited due to low yields and high-cost stemming from the engineering expertise and manual skill required to design and fabricate them. Additive manufacturing techniques making use of digital modularity can reduce the expertise and skill required while increasing functionality with multi-material components. We report on our work evaluating the biocompatibility of 3D-printed cell culture devices with various materials and surface modifications. OOC devices were fabricated from Cyclic Olefin Co-polymer (COC) using fused filament fabrication. Additional components were fabricated from silicone and chitosan with extrude and cure printing and electrospinning to provide cell-culture substrates that better mimic native tissues. To further enhance the material biocompatibility and promote cell adhesion, we treated surfaces with corona plasma and polydopamine surface coating.To evaluate the biocompatibility of the materials and surface modifications used in our composite devices, we employ and optimize live/dead viability assay procedures using a combination of highly sensitive fluorescent dyes (Calcein Blue AM and 7-AAD) in 3D-printed COC cell culture devices in vitro. These experiments and the resulting protocols provide a comprehensive method to assess novel materials and cell culture device configurations. The work also provided research-level feedback on the usability of the devices which led to iterative redesign which will be reported. Both outcomes set the foundation for the future construction of affordable, biocompatible, and functional organ-on-chip (OOC) systems manufactured using COC. The successful fabrication of biocompatible 3D-printed cell culture devices using COC and additional materials presented by this project may overcome the manufacturing limitations of OOC using bioengineering strategies, which enables for future mass-production of various OOC systems.
Russell K. Pirlo
Primary Advisor's Department
Chemical and Materials Engineering
Stander Symposium project, School of Engineering
United Nations Sustainable Development Goals
Good Health and Well-Being; Industry, Innovation, and Infrastructure
"Assessing and Improving the Biocompatibility and Usability of Composite Additively Manufactured Organ-On-Chip Devices" (2022). Stander Symposium Projects. 2722.