Investigating the Role of CodY in Regulating hly Transcript Level in Response to Propionate
Listeria monocytogenes is a deadly foodborne pathogen with a variety of virulence factors. During its transmission from food products to the human intestines, L. monocytogenes needs to respond accurately to the changes in the environmental signals to coordinate the expression of the appropriate virulence factors. Through previous research in the lab, it was identified that the transcription factor CodY plays a key role in regulating the production of listeriolysin O (LLO), a virulence factor necessary for L. monocytogenes to establish an intracellular infection, in response to propionate. To confirm that this regulation takes place at the transcriptional level, I conducted quantitative reverse transcription PCR experiments to determine the transcript level of hly, the gene that codes for LLO, in response to propionate. I also compared the propionate response in wildtype and ΔcodY to determine whether the transcriptional response was dependent on the transcription factor CodY. With three independent trials, preliminary results showed that hly transcript levels were affected by propionate treatments. Further analysis will reveal whether CodY is involved in the propionate response.
Course Project 202310 BIO 421 P2
Primary Advisor's Department
Stander Symposium, College of Arts and Sciences
"Investigating the Role of CodY in Regulating hly Transcript Level in Response to Propionate" (2023). Stander Symposium Projects. 2892.