Structure-function analysis of Defective proventriculus (Dve) in Drosophila melanogaster eye growth and development
Anuradha Chimata Venkatakrishnan
Axial patterning is required to delineate Antero-Posterior (AP), Dorso-Ventral (DV), and Proximo-Distal (PD) axes in a sequential manner to transform a monolayer organ primordium to a three-dimensional organ. During eye development, DV axis is the first lineage restriction event and any deviation results in developmental birth defects. During this process, generation and establishment of morphogen gradient plays important roles such as negative regulator of eye development, growth and determination of eye versus head fate. Previously, we have identified defective proventriculus (dve, an ortholog of SATB1) as a novel dorsal patterning gene that regulates wingless (wg) transcription. Loss-of-function of dve results in dorsal eye enlargement and decrease in Wg, while gain-of-function of dve results in eye suppression and increased wg. In addition to eye versus head fate, SATB1, the human ortholog of dve is highly upregulated in cancers and is known to activate various signaling pathways to trigger growth. Here we present our structure-function analysis to determine the domains of Dve required for growth regulation and Wg mediated determination of eye versus head fate. Dve has a ULD domain for tetramerization, two HOX domains for DNA binding and a PPP4R2 domain for H2AFX dephosphorylation. We have developed several transgenic fly lines, which will allow us to ectopically induce expression of the specific Dve protein domain(s) alone or in combination and assay their effect in the eye. Our preliminary data suggests that HOX domain alone can induce wg expression and exhibit weak eye suppression phenotype, but it is not enough to fully exhibit Dve mediated eye suppression function during development. We will present data from our studies to dissect the growth versus eye fate function of Dve protein domain(s) using this structure-function analysis. Understanding this can increase our knowledge of which domains might be dispensable or pathogenic in human development or disease.
Amit Singh, Madhuri Kango-Singh
Primary Advisor's Department
Stander Symposium, College of Arts and Sciences
"Structure-function analysis of Defective proventriculus (Dve) in Drosophila melanogaster eye growth and development" (2023). Stander Symposium Projects. 3072.