Ben Klocke


Presentation: 1:00-1:20 p.m., Science Center 146



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Calcium (Ca2+) ions comprise a critical second messenger involved in a constellation of cellular processes, including cell proliferation and death, metabolism, and gene expression. Naturally, neuronal Ca2+ signaling is vital in processes such as long-term potentiation (LTP) and synaptic transmission, among others. A critical key component of the neuronal Ca2+ handling toolkit is the sarco/endoplasmic reticulum Ca2+ ATPase 2 (SERCA) pump. Further, dysfunction of SERCA2 has been implicated in many brain pathologies. Interestingly, our group has identified a regulator of SERCA2 being expressed in the mouse thalamus; global genetic ablation of this gene (GKO) in mice results in an ADHD-like behavioral phenotype. Our lab has recently developed a novel Cre-LoxP conditional knockout mouse model (cKO) to assess the effects of this Ca2+-handling player in the brain. Behavioral analysis of cKO mice showed that they exhibit locomotor hyperactivity and altered sleep architecture. Further, testing in the 5-choice serial reaction time task (5-CSRTT), a behavioral paradigm used to assess attentional capacity and impulsivity, showed that cKO mice exhibit impulsivity. Altogether, these findings strongly support a novel role for this Ca2+-handling player in the regulation of brain function and behavioral processes.

Publication Date


Project Designation

Graduate Research

Primary Advisor

Pothitos Pitychoutis

Primary Advisor's Department



Stander Symposium, College of Arts and Sciences

Institutional Learning Goals

Scholarship; Practical Wisdom

Using Transgenic Mouse Models to Dissect a Novel Calcium Signaling Pathway in the Mouse Brain