Studying the Role of Hedgehog Signaling Pathway in Dorso-Ventral Patterning
Anuradha Chimata Venkatakrishnan, Soumya Bajpai; other authors: Madhuri Kango-Singh, Amit Singh
During organogenesis, 3-dimentional organs arise from a monolayer of cells and this requires axial patterning to establish Dorsal-Ventral (DV), Anterior-Posterior (AP) and Proximal-Distal (PD) axes. Among them, the DV axis is the first to form during eye development. Morphogens have also been associated with a multitude of developmental processes, including organ patterning and the control of organ size. The Hedgehog (Hh) family of molecules play an important role in eye development as it regulates formation of the retina in Drosophila by orchestrating a differentiation wave that allows for the rapid and precise differentiation of the fly retina. We wanted to understand how Hh signaling interacts with a previously identified dorsal selector gene, defective proventriculus (dve). Dve is a K-50 homeodomain containing transcription factor, a Drosophila ortholog of human SATB1. We know that loss-of-function of dve results in dorsal eye enlargement while gain-of-function results in complete eye suppression. We wanted to understand how this transcription factor can regulate Hh signaling pathway that is crucial for patterning, growth and eye development. Here, we present our results from modulating Hh signaling pathway in dve expression domain and how it affects eye development.
Amit Singh, Madhuri Kango-Singh
Primary Advisor's Department
Stander Symposium, College of Arts and Sciences
Institutional Learning Goals
"Studying the Role of Hedgehog Signaling Pathway in Dorso-Ventral Patterning" (2023). Stander Symposium Projects. 3118.