i-TRACE RNA interference-based reporter system to distinguish spatiotemporal gene expression in real time versus lineage cells in Drosophila
In all cells, dynamic gene expression along the spatiotemporal axis proves to be vital in any organism’s development. These changes are responsible for cellular responses to stimuli as well as execution of sequential developmental programs. This execution is highly regulated and highly specific. In the development of the Drosophila eye, multiple genes are expressed at various times in order to regulate target genes. The interest of this project is to is to analyze the exact positions of this dynamic expression of certain genes involved in the fruit fly’s eye development. The i- TRACE (RNAi Technique for Real- time And Clonal Expression) system is being used to observe the small yet active changes in expression patterns. The Gal4/UAS, FLP/FRT, RNAi and fluorescent reporters are used in combination with the i-TRACE system to assess gene expression. Real time expression that is Gal4 mediated is observed in the presence of a red fluorescent protein known as RFP. Similarly, any lineage cells are marked by the presence of green fluorescent protein, or GFP, which functions independently of Gal4. Finally, GFP-RNAi expression, which is Gal4 mediated, exists in cells that have either currently or recently expressed the gene. Because of this, observations in minute changes in expression are able to be observed as marked by a loss of GFP. Here, we present the expression data of some of the genes that play an important role during Drosophila eye development.
Primary Advisor's Department
Stander Symposium, College of Arts and Sciences
Institutional Learning Goals
"i-TRACE RNA interference-based reporter system to distinguish spatiotemporal gene expression in real time versus lineage cells in Drosophila" (2023). Stander Symposium Projects. 3134.