Role of ZFP36L1 in suppressing human coronavirus replication

Role of ZFP36L1 in suppressing human coronavirus replication

Presenter(s)

Andrew Villasenor, Tooba Shafeeque Ahmed Momin, Malabika Bhowmik

Comments

Presentation: 11:40 a.m.-12:00 p.m., Science Center 150

Description

ZFP36L1 is a CCCH-type zinc figure protein (ZFP) where zinc ions coordinate the protein structure in a tetrahedral geometry by binding to cystine-cystine or cysteine-histidine amino acids. ZFP36L1’s unique structure enables it to interact with a wide variety of molecules including RNA; thus, it could modulate several cellular processes including virus replication. Several CCCH-type ZFPs have shown their antiviral efficacy against various DNA and RNA viruses. However, the role of ZFP36L1 in the human coronavirus is little explored. We used human coronavirus (HCoV)-OC43 to determine the role of ZFP36L1 on its replication. We overexpressed and knockdown ZFP36L1 in HCT-8 cells individually using lentivirus transduction. Wild type, ZFP36L1 overexpressed, and ZFP36L1 knockdown cells were each infected with HCoV-OC43, and the virus titer in each cell line was measured over 96 hours post-infection (p.i.). Our results show that HCoV-OC43 replication was significantly reduced with ZFP36L1 overexpression while ZFP36L1 knockdown significantly enhanced virus replication. ZFP36L1 knockdown HCT-8 cells started producing infectious virus at 48 hours p.i. which was an earlier timepoint as compared to wild-type and ZFP36L1 overexpressed cells. Wild-type and ZFP36L1 overexpressed HCT-8 cells started producing infectious virus at 72 hours p.i. Overall, the current study showed that overexpression of ZFP36L1 suppressed human coronavirus (OC43) production

Publication Date

4-19-2023

Project Designation

Graduate Research

Primary Advisor

Mrigendra Rajput, Amit Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium, College of Arts and Sciences

Institutional Learning Goals

Scholarship

Role of ZFP36L1 in suppressing human coronavirus replication

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