Determining the role of ZFP36L1 in reducing norovirus replication and norovirus-induced damage in the cells
Paige Howland, Luis Martin, Madison Richardson, Tooba Shafeeque Ahmed Momin, Abiageal Newell
Norovirus is a positive sense, non-enveloped virus. This virus is one of the most common causes of acute viral gastroenteritis (stomach flu) in humans. Norovirus is resistant to commonly used disinfectants such as 70% ethanol and currently, we do not have any vaccine against this virus. In the current study, we explored the role of ZFP36L1, a CCCH-type zinc figure protein (ZFP) in suppressing norovirus replication using murine norovirus as a model. ZFP36L1 was overexpressed or knockdown in RAW 264.7 cells. Wild type, ZFP36L1 overexpressed or ZFP36L1 knockdown RAW 264.7 cells were infected with murine norovirus. Virus titer in those cells were measured at 24 hours post-infection (p.i.). Our results show that ZFP36L1 knockdown significantly enhanced the virus titer as well as the cytopathic effect in the cells. ZFP36L1 overexpression reduced the cytopathic effect in the cells as compared to the wild-type and ZFP36L1 knockdown cells. ZFP36L1 overexpression is showing trends in reducing the virus titer which needs to be confirmed for statistical significance with a going study.
Primary Advisor's Department
Stander Symposium, College of Arts and Sciences
Institutional Learning Goals
"Determining the role of ZFP36L1 in reducing norovirus replication and norovirus-induced damage in the cells" (2023). Stander Symposium Projects. 3186.