Yorkie dependent transcriptional network promotes tumor growth
Arushi Rai; other authors: Indrayani Waghmare, Amit Singh, Madhuri Kango-Singh
The Hippo pathway effector, Yorkie (Yki) is a key mediator of signaling interactions and transcriptional addictions in tumor cells and presents an attractive opportunity to study transcriptional dependencies in cancer cells. The RasV12 scrib-/- tumor mosaic model, a well-established model, shows activation of oncogenic Ras in the background of impaired apical-basal polarity. Previously, we have shown that in RasV12, scrib-/- cells Wingless (Wg) act upstream of Caspases, JNK and Yki and via its canonical and non-canonical pathways to interact with Yki to regulate the development and cancer growth. Our goal is to understand further how the two evolutionarily conserved signaling pathways i.e., Hippo and Wingless crosstalk and interact with each other to regulate tumor growth using the RasV12, scrib-/- tumor model in Drosophila imaginal discs. Our data showed that the wg transcriptional reporters and wg transcript levels both are upregulated in RasV12, scrib-/- cells. In other contexts, wg is shown as a transcriptional target of Yki. Therefore, we will test for (a) the effects of Yorkie protein, the main effector molecule of the Hippo pathway, on the transcription and expression of Wg and other Wg pathway components by reporter assays, and qRT-PCR- based approaches, and (b) the effect of wg pathway components (frizzled, TCF) on the intrinsic wingless signaling and also the growth of RasV12, scrib-/- tumor (c)the effect of feedback interactions that promote tumorigenesis using genetic epistasis-, and immunohistochemistry-based approaches. Here, we present our progress on the organization of the molecular network involving Wingless and Yorkie.
Madhuri Kango-Singh, Amit Singh
Primary Advisor's Department
Stander Symposium, College of Arts and Sciences
Institutional Learning Goals
Community; Diversity; Faith
"Yorkie dependent transcriptional network promotes tumor growth" (2023). Stander Symposium Projects. 3209.
Presentation: 1:15-2:30 p.m., Kennedy Union Ballroom