Understanding the Epithelial to Mesenchymal Transition of Glioblastoma Multiforme on a Microfluidic Model

Understanding the Epithelial to Mesenchymal Transition of Glioblastoma Multiforme on a Microfluidic Model

Presenter(s)

Khadija Fatima

Comments

1:00-1:20, Kennedy Union 207

Description

Glioblastoma Multiforme (GBM) is one of the most aggressive and fatal forms of brain cancer. Despite multiple advances in treatment, median survival is only about 15 months upon diagnosis. Hence, numerous studies have been conducted to further our understanding of GBM tumor biology and the mechanisms underlying its malignancy. I am particularly interested in the Epithelial to Mesenchymal Transition (EMT) of GBM cells, which has been shown to enhance their migratory and invasive capabilities. In this study, I aim to investigate the specific effect of the TGFβ inhibitor, SB-431542, on EMT as well as other aberrant outcomes. Using the non-adherent method and microfluidic technology, I can generate a model of three-dimensional GBM spheroids surrounded by physical constrictions, which mimics the GBM tumor microenvironment. The TGFβ inhibitor will be introduced, and the resulting sprouting and migrating behaviors of the invasive cells will be quantified. To enhance the generalizability of my findings, I have expanded my research to include multiple GBM cell lines. I hypothesize a detectable decrease in the level of invasion among the treated spheroids compared to the control. The findings of this proposed study will broaden our knowledge of the complexity of EMT and its role in GBM cancer development and metastasis. Moreover, I hope my work will provide the means to improve therapies that can inhibit cancer cell dissemination in GBM patients.

Publication Date

4-23-2025

Project Designation

Honors Thesis

Primary Advisor

Loan T. Bui

Primary Advisor's Department

Biology

Keywords

Stander Symposium, College of Arts and Sciences

Institutional Learning Goals

Scholarship

Understanding the Epithelial to Mesenchymal Transition of Glioblastoma Multiforme on a Microfluidic Model

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