Defective proventriculus interacts with Yorkie to regulate growth in the developing eye

Defective proventriculus interacts with Yorkie to regulate growth in the developing eye

Presenter(s)

Rohith Nanjundaiah

Comments

10:40-11:00, Kennedy Union 222

Description

The developing eye of Drosophila is a well-established model for studying developmental genetic processes and growth regulation. Our long-term goal is to understand the molecular basis of Dorsal-Ventral patterning and growth in the eye by interactions of the dorsal selector genes and growth regulatory genes. We recently identified defective proventriculus (dve) as a candidate for dorsal-ventral eye patterning that acts as a transcriptional regulator. Gain of function of Dve, results in eye suppression, while loss of function of dve, exhibits dramatic eye enlargement phenotypes. This raised an interesting question, whether the dorsal patterning gene dve, apart from its main function of specifying cells fate, plays a dual role in regulating growth during eye development in Drosophila? We hypothesized that Dve may interact with the Hippo growth regulatory pathway to control patterning and growth of the eye. We tested the interactions between Hippo pathway and dorsal-ventral patterning using the GAL4-UAS system and MARCM. To investigate whether yki is involved in dve domain growth, we used GAL4 drivers in the eye like Dve Gal4 to drive the spatiotemporal expression of transgenes in the dorsal eye, and Ey-Gal4 and GMR-Gal4 to test the epistasis of Dve and Yki before and after MF formation in the larval eye antennal disc for yki target genes ( ex, diap1 and hth) and dve target genes mirr, wingless (wg) (a known and conserved Hippo downstream target) for Dve and Yki mediated effects using reporter assays, clonal analysis and qRT-PCR- based approaches; and our results will be discussed.

Publication Date

4-23-2025

Project Designation

Independent Research

Primary Advisor

Madhuri Kango-Singh, Amit Singh

Primary Advisor's Department

Biology

Keywords

Stander Symposium, College of Arts and Sciences

Institutional Learning Goals

Practical Wisdom; Scholarship

Defective proventriculus interacts with Yorkie to regulate growth in the developing eye

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