Caroline Wise



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In response to the current global energy crisis, biofuels have become a viable renewable energy solution and require a carbohydrate source to begin their production. One such carbohydrate source option is biomass, which is comprised of complex sugars that can be broken down into simple sugars and then fermented for the production of bioethanol. The bacterium called Caldicellulosiruptor saccharolyticus contains many glycoside hydrolase enzymes that have the potential for metabolizing the complex sugars in several carbohydrate sources, including those in biomass. This project is focused on the cloning of the Csac_2410 gene from C. saccharolyticus, expression of the gene as a protein, purification of the protein, and biochemical characterization of the protein. The biochemical characterization determines the substrate specificity, pH optima and temperature optima of Csac_2410, and the results are used to determine the effectiveness of Csac_2410 in metabolizing complex sugars for the upstream processing of biofuels.

Publication Date


Project Designation

Honors Thesis

Primary Advisor

Donald A. Comfort

Primary Advisor's Department

Chemical and Materials Engineering


Stander Symposium project


Arts and Humanities | Business | Education | Engineering | Life Sciences | Medicine and Health Sciences | Physical Sciences and Mathematics | Social and Behavioral Sciences

Purification and Biochemical Characterization of a Xylanolytic Glycoside Hydrolase from Caldicellulosiruptor saccharolyticus