Madison Frazier Bourbon, Robert Forrest Uhrig



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Silver nanoparticles (AgNPs) are utilized in many different applications, such as an antibacterial agents or as protective coating against ultraviolet light. However, these AgNPs are known to cause potentially harmful biological effects, including toxicity, induction of stress, and immune activation. In this study, the effects of AgNPs on a human lung cell model were examined within both a static and dynamic environment. Most NP-based research is carried out in static environments, but do not accurate reflect dynamic physiological conditions. Dynamic fluid movement was introduced to the cell culture through the use of a multi-channel peristaltic pump. To further characterize the influence of fluid movement, two different sized AgNPs were tested, 5 nm and 50 nm. The AgNPs were then introduced to the lung cells, under either static or dynamic conditions for a duration of 24-hours. Following this exposure, the cells underwent evaluation for NP deposition, cell viability, cell stress, and inflammatory responses. The results indicated that biological responses were dependent on the delivered NP dosage, which was substantially diminished in a dynamic environment. This work highlights the necessity of carrying out NP-evaluation studies in a cell system that more closely mimics a true physiological environment.

Publication Date


Project Designation

Honors Thesis - Undergraduate

Primary Advisor

Kristen K. Comfort

Primary Advisor's Department

Chemical and Materials Engineering


Stander Symposium project

The Impact of a Dynamic Environment on Deposition and Cellular Response to Silver Nanoparticles