Honors Theses

Title

Boron Dipyrromethene Dyes Covalently Bound to Bovine Serum Albumin: A Simplified Route to Fluorescent Dye-Protein Conjugates

Advisor

Shawn Swavey

Department

Chemistry

Publication Date

4-1-2022

Document Type

Honors Thesis

Abstract

Fluorescent probes/dyes have proved critical to disentangling complex cellular functions leading to the discovery of therapeutics for a variety of diseases. Visualization of the smallest molecular functions within a cell is best accomplished with probes capable of not only localization at specific cellular regions but emission of light within the near infrared (NIR) portion of the electromagnetic spectrum (i.e., wavelengths from 650 nm to 900 nm). Novel fluorescent BODIPY dyes with absorption and emission propterites across the electromagnetic spectrum were synthesized using a simplified route. These BODIPY dyes were then bound to NHS esters using standard coupling reagents. Following their purification, BODIPY-NHS dyes were successfully bound to BSA protein. Swavey Lab is currently working in collaboration for these dyes to be functionally tested for their potential use as fluorescent probes in immunohistochemical experiments to visualize neural cells in the mouse brain.

Permission Statement

This thesis is not available in the repository or through interlibrary loan; to view it in person, visit the University Archives by telephone at 937-229-4256 or via email at archives@udayton.edu.

Keywords

Undergraduate research

Disciplines

Chemistry

This document is currently not available here.


Share

COinS