Shawn Swavey, Ph.D.
A new single molecule 4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) dye designed to conjugate to the peripheral amines of proteins has been synthesized. Detailed synthesis involving a new and simple two step technique is presented. Synthesis of the carboxylic acid substituted BODIPY dye is characterized by absorption and emission spectroscopy. The new dye is reacted with N- hydroxysuccinimide to give a reactive BODIPY dye toward amine groups of bovine serum albumin. The BODIPY-N-succinimide ester was evaluated by absorption and emission spectroscopy. Covalent attachment of the BODIPY-NHS-ester with bovine serum albumin was conducted in an eppendorf tube in a phosphate buffer at a pH of 7.0. Purification by column chromatography and evaluation of the solution suggests a degree of labeling of only 50% of the bovine serum albumin.
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Hess, Amy, "Boron Dipyrromethene (BODIPY) Dye Covalently Bound to Bovine Serum Albumin (BSA)" (2023). Honors Theses. 401.