Document Type

Article

Publication Date

2-4-2020

Publication Source

Plos One

Abstract

Bioethanol production from lignocellulosic biomass has received increasing attention over the past decade. Many attempts have been made to reduce the cost of bioethanol produc- tion by combining the separate steps of the process into a single-step process known as consolidated bioprocessing. This requires identification of organisms that can efficiently decompose lignocellulose to simple sugars and ferment the pentose and hexose sugars lib- erated to ethanol. There have been many attempts in engineering laboratory strains by add- ing new genes or modifying genes to expand the capacity of an industrial microorganism. There has been less attention in improving bioethanol-related processes utilizing natural variation existing in the natural ecotypes. In this study, we sought to identify genomic loci contributing to variation in saccharification of cellulose and fermentation of glucose in the fermenting cellulolytic fungus Neurospora crassa through quantitative trait loci (QTL) analy- sis. We identified one major QTL contributing to fermentation of glucose and multiple puta- tive QTL’s underlying saccharification. Understanding the natural variation of the major QTL gene would provide new insights in developing industrial microbes for bioethanol production.

ISBN/ISSN

1932-6203

Document Version

Published Version

Comments

This open-access article is provided for download in compliance with the publisher’s policy on self-archiving. To view the version of record, use the DOI: https://doi.org/10.1371/journal.pone.0221737

Publisher

Public Library Science

Volume

15

Peer Reviewed

yes

Issue

2


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