Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometric Method for the Determination of Artemisinin in Rat Serum and its Application in Pharmacokinetics

Author(s)

Lie Li, University of Mississippi
Deepthi Pabbisetty, University of Mississippi
Paulo Carvalho, University of Mississippi
Mitchell A. Avery, University of Mississippi
John S. Williamson, University of DaytonFollow
Bonnie A. Avery, University of Mississippi

Document Type

Article

Publication Date

5-2008

Publication Source

Journal of Chromatography B

Abstract

A rapid and sensitive ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to quantify artemisinin in rat serum. The lower limit of quantification (LLOQ) was 4 ng/mL. The calibration curve was linear from 4 ng/mL to 10,000 ng/mL (R = 0.998). The assay was based on the selected reaction monitoring (SRM) transitions at m/z 305.4–151.10 for artemisinin and m/z 335.2–163.10 for arteether (internal standard). The artemisinin and internal standard can be separated from endogenous interferences in rat serum. Inter- and intra-day assay variation was less than 15%. The extraction recoveries ranged from 80.0 to 107.3% at the three concentrations (5000, 2000, and 200 ng/mL). This method was successfully applied to pharmacokinetic studies of artemisinin after intravenous and oral administration to rats.

Inclusive pages

131–137

ISBN/ISSN

1570-0232

Comments

Permission documentation is on file.

Copyright

Copyright © 2008, Elsevier

Publisher

Elsevier

Volume

867

Issue

1

Peer Reviewed

yes

eCommons Citation

Li, Lie; Pabbisetty, Deepthi; Carvalho, Paulo; Avery, Mitchell A.; Williamson, John S.; and Avery, Bonnie A., "Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometric Method for the Determination of Artemisinin in Rat Serum and its Application in Pharmacokinetics" (2008). Office for Research Publications and Presentations. 35.
https://ecommons.udayton.edu/ofr_pub/35