Document Type
Article
Publication Date
9-10-2024
Publication Source
Antibiotics
Volume
13
Publisher
MDPI
ISBN/ISSN
2079-6382
Peer Reviewed
yes
Abstract
Abstract: We analyzed the whole genome sequences (WGS) and antibiograms of 35 Enterobacter isolates, including E. hormaechei and E. asburiae, and the recently described E. bugandensis, E. kobei, E. ludwigii, and E. roggenkampii species. Isolates were obtained from human blood and urinary tract infections in patients in the United States. Our goal was to understand the genetic diversity of antimicrobial resistance genes and virulence factors among the various species. Thirty-four of 35 isolates contained an AmpC class blaACT allele; however, the E. roggenkampii isolate contained blaMIR-5. Of the six Enterobacter isolates resistant to ertapenem, imipenem, and meropenem, four harbored a carbapenemase gene, including blaKPC or blaNDM. All four isolates were mCIM-positive. The remaining two isolates had alterations in ompC genes that may have contributed to the resistance phenotype. Interpretations of cefepime test results were variable when disk diffusion and automated broth microdilution results were compared due to the Clinical Laboratory and Standards Institute use of the “susceptible dose-dependent” classification. The diversity of the blaACT alleles paralleled species identifications, as did the presence of various virulence genes. The classification of recently described Enterobacter species is consistent with their resistance gene and virulence gene profiles.
Keywords
Enterobacter; sequencing; AmpC beta-lactamases; carbapenemases; antimicrobial resistance
Disciplines
Bacteriology | Microbiology
eCommons Citation
Tenover, Fred C. and Tickler, Isabella A., "Genomic Analysis of Enterobacter Species Isolated from Patients in United States Hospitals" (2024). Guidelines, Recommendations, and Other Resources on Antimicrobial Resistance. 2.
https://ecommons.udayton.edu/amr_resources/2
Comments
Link to journal website https://doi.org/10.3390/antibiotics13090865