Excretion and Conservation of Glycerol, and Expression of Aquaporins and Glyceroporins, During Cold Acclimation in Cope's Gray Tree Frog Hyla Chrysoscelis

Document Type

Article

Publication Date

1-2007

Publication Source

American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Abstract

Cope's gray tree frog Hyla chrysoscelis accumulates glycerol during cold acclimation. We hypothesized that, during this process, gray tree frogs adjust renal filtration and/or reabsorption rates to retain accumulated glycerol. During cold acclimation, plasma concentrations of glycerol rose >200-fold, to 51 mmol/l. Although fractional water reabsorption decreased, glomerular filtration rate (GFR) and, consequently, urine flow wereXenopus oocyte expression system. HC-1, an AQP1-like water channel conferring low glycerol permeability, is expressed ubiquitously in warm- and cold-acclimated tissues. HC-2, a water channel most similar to AQP2, is primarily expressed in organs of osmoregulation. HC-3, which is most similar to AQP3, is functionally characterized as a GLP, with low permeability to water but high permeability to glycerol. Aspects of expression levels and functional characteristics varied between cold and warm conditions for each of the three AQPs, suggesting a complex pattern of involvement in osmoregulation related to thermal acclimation.

Inclusive pages

R544-R555

ISBN/ISSN

0363-6119

Comments

Permission documentation is on file.

This research was supported in part by National Science Foundation Research Grant IOB-0517301 to D.L. Goldstein and C.M. Krane.

Publisher

American Physiological Society

Volume

292

Peer Reviewed

yes

Issue

1

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