Document Type

Article

Publication Date

4-19-2011

Publication Source

Molecular Vision

Abstract

Purpose: To evaluate the effects of complement employing a mouse model for secondary cataract. Methods: The role of complement receptor C5a (CD88) was evaluated after cataract surgery in mice. An antagonist specific to C5a receptor was administered intraperitoneally to mice. Epithelial to mesenchymal transition (EMT) was evaluated by alpha-smooth muscle actin (α-SMA) staining and proliferation by bromodeoxyuridine (5-bromo-2'- deoxyuridine, BrdU) incorporation. Gene expression patterns was examined by microarray analysis and quantitative polymerase chain reaction (QPCR). Results: We found that administration of a C5aR antagonist in C57BL/6J mice decreases EMT, as evidenced by α-SMA expression, and cell proliferation. Gene expression by microarray analysis reveals discreet steps of gene regulation in the two major stages that of EMT and lens fiber differentiation in vivo. A hallmark of the microarray analysis is that the antagonist seems to be a novel stage-specific regulator of crystallin genes. At week two, which is marked by lens fiber differentiation genes encoding 12 crystallins and 3 lens-specific structural proteins were severely down-regulated. Conclusions: These results suggest a possible therapeutic role of an antagonist to C5aR in preventing secondary cataracts after surgery. Also these results suggest that crystallin gene expression can be regulated by pro-inflammatory events in the eye.

Inclusive pages

949-964

ISBN/ISSN

1090-0535

Document Version

Published Version

Publisher

Molecular Vision

Volume

17

Peer Reviewed

yes

Issue

105

Keywords

Molecular Vision, Article, Posterior Capsule Opacification, Gene-Expression, Microarray Data, Regeneration, Model

Download

Included in

Biology Commons

Share

COinS