Domain Specific Genetic Mosaic System in the Drosophila Eye

Author(s)

Meghana Tare, University of Dayton
Oorvashi Roy Puli, University of DaytonFollow
Michael Moran, University of Dayton
Madhuri Kango-Singh, University of DaytonFollow
Amit Singh, University of DaytonFollow

Document Type

Article

Publication Date

1-2013

Publication Source

Genesis: The Journal of Genetics and Development

Abstract

Genetic mosaic approach is commonly used in the Drosophila eye by completely abolishing or misexpressing a gene within a subset of cells to unravel its role during development. Classical genetic mosaic approach involves random clone generation in all developing fields. Consequently, a large sample size needs to be screened to generate and analyze clones in specific domains of the developing eye. To address domain specific functions of genes during axial patterning, we have developed a system for generating mosaic clones by combining Gal4/UAS and flippase (FLP)/FRT system which will allow generation of loss-of-function as well as gain-of-function clones on the dorsal and ventral eye margins. We used the bifid-Gal4 driver to drive expression of UAS-FLP. This reagent can have multiple applications in (i) studying spatio-temporal function of a gene during dorso-ventral (DV) axis specification in the eye, (ii) analyzing genetic epistasis of genes involved in DV patterning, and (iii) conducting genome wide screens in a domain specific manner.

Inclusive pages

68–74

ISBN/ISSN

1526-954X

Comments

Permission documentation is on file.

Copyright

Copyright © 2013, Wiley Periodicals

Publisher

Wiley Periodicals

Volume

51

Peer Reviewed

yes

Issue

1

eCommons Citation

Tare, Meghana; Puli, Oorvashi Roy; Moran, Michael; Kango-Singh, Madhuri; and Singh, Amit, "Domain Specific Genetic Mosaic System in the Drosophila Eye" (2013). Biology Faculty Publications. 161.
https://ecommons.udayton.edu/bio_fac_pub/161