Document Type
Article
Publication Date
9-2004
Publication Source
Journal of Bacteriology
Abstract
Bordetella species utilize the BvgAS (Bordetella virulence gene) two-component signal transduction system to sense the environment and regulate gene expression among at least three phases: a virulent Bvg+ phase, a nonvirulent Bvg− phase, and an intermediate Bvgi phase. Genes expressed in the Bvg+ phase encode known virulence factors, including adhesins such as filamentous hemagglutinin (FHA) and fimbriae, as well as toxins such as the bifunctional adenylate cyclase/hemolysin (ACY). Previous studies showed that in the Bvgi phase, FHA and fimbriae continue to be expressed, but ACY expression is significantly downregulated. In this report, we determine that Bordetella bronchiseptica can form biofilms in vitro and that the generation of biofilm is maximal in the Bvgi phase. We show that FHA is required for maximal biofilm formation and that fimbriae may also contribute to this phenotype. However, expression of ACY inhibits biofilm formation, most likely via interactions with FHA. Therefore, the coordinated regulation of adhesins and ACY expression leads to maximal biofilm formation in the Bvgi phase in B. bronchiseptica.
Inclusive pages
5692-5698
ISBN/ISSN
0021-9193
Document Version
Published Version
Copyright
Copyright © 2004, American Society for Microbiology
Publisher
American Society for Microbiology
Volume
186
Peer Reviewed
yes
Issue
17
eCommons Citation
Irie, Yasuhiko; Mattoo, Seema; and Yuk, Ming H., "The Bvg Virulence Control System Regulates Biofilm Formation in Bordetella bronchiseptica" (2004). Biology Faculty Publications. 199.
https://ecommons.udayton.edu/bio_fac_pub/199
Included in
Biology Commons, Biotechnology Commons, Cell Biology Commons, Genetics Commons, Microbiology Commons, Molecular Genetics Commons
Comments
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