Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in 'Pseudomonas aeruginosa'
Document Type
Book Chapter
Publication Date
2017
Publication Source
c-di-GMP Signaling: Methods and Protocols
Abstract
3′,5′-cyclic diguanosine monophosphate (cyclic di-GMP) is a bacterial secondary messenger molecule that regulates many important cellular activities and behaviors, such as motility and biofilm formation. While mass spectrometry protocols for quantitative analyses of intracellular cyclic di-GMP concentrations have been developed, they are time intensive, expensive, low-throughput, and incapable of directly monitoring dynamic changes in vivo. In this protocol, we provide a Pseudomonas aeruginosa-specific detailed methodology to assay the intracellular levels of cyclic di-GMP using biological reporters.
ISBN/ISSN
978-1-4939-7239-5
Copyright
Copyright © 2017, Springer
Publisher
Springer
eCommons Citation
Borlee, Bradley R.; Borlee, Grace I.; Martin, Kevin H.; and Irie, Yasuhiko, "Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in 'Pseudomonas aeruginosa'" (2017). Biology Faculty Publications. 217.
https://ecommons.udayton.edu/bio_fac_pub/217
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