Document Type
Article
Publication Date
2017
Publication Source
Drosophila Information Service
Abstract
The objective of this teaching note is to develop a laboratory exercise, which allows students to get a hands-on experience of a molecular biology technique to analyze gene expression. The short duration of the biology laboratory for an undergraduate curriculum is the biggest challenge with the development of new labs. An important part of cell biology or molecular biology undergraduate curriculum is to study gene expression. There are many labs to study gene expression in qualitative manner. The commonly used reporter gene expression studies are primarily qualitative. However, there is no hands-on experience exercise to quantitatively determine gene expression. Therefore, it is necessary to design a laboratory exercise that enables the students to carry out cell or molecular biological assays in the desired time. Here we report a laboratory where we can introduce students to gene expression using the real time Quantitative Polymerase Chain Reaction (RT-qPCR) by comparative CT method to analyze expression of genes in Drosophila tissues.
Inclusive pages
225-230
ISBN/ISSN
0070-7333
Document Version
Published Version
Copyright
Copyright © 2017, Drosophila Information Service
Publisher
Drosophila Information Service
Volume
100
Keywords
Drosophila melanogaster, eye, real time quantitative PCR, gene expression
eCommons Citation
Mehta, Abijeet Singh and Singh, Amit, "Real-Time Quantitative PCR to Demonstrate Gene Expression in an Undergraduate Lab" (2017). Biology Faculty Publications. 237.
https://ecommons.udayton.edu/bio_fac_pub/237
Included in
Biology Commons, Biotechnology Commons, Cell Biology Commons, Genetics Commons, Microbiology Commons, Molecular Genetics Commons
Comments
Document is made available for download with the permission of the publisher. Permission documentation is on file.