Document Type
Article
Publication Date
1-1993
Publication Source
Electrophoresis
Abstract
In this paper we describe a two-dimensional gel database of the regenerating newt limb. Protein synthesis was compared in the intact limb, in the 1-week regenerating limb, representing the dedifferentiation stage, and in the 2-week regenerating limb, representing the formation of the blastema. This comparative database provided data on differential expression of about 800 proteins during the process of limb regeneration. In addition, a map has been generated for these proteins for future guidance in characterizing further new, unknown proteins. The overall expression patterns of the proteins indicated that the dedifferentiation stage was marked by down-regulation of most proteins, while the blastema formation was marked by the appearance of many new proteins. The potential use of such a database in isolating factors involved during limb regeneration is discussed.
Inclusive pages
148-156
ISBN/ISSN
0173-0835
Document Version
Published Version
Copyright
This is a copy of an article published in Electrophoresis © 1993 copyright Vch Publishers Inc.
Publisher
Vch Publishers Inc
Volume
14
Peer Reviewed
yes
Issue
1
Keywords
Vch Publishers Inc, Article
eCommons Citation
Tsonis, Panagiotis A., "A Comparative 2-Dimensional Gel Protein Database of the Intact and Regenerating Newt Limbs" (1993). Biology Faculty Publications. 26.
https://ecommons.udayton.edu/bio_fac_pub/26
Included in
Biochemistry Commons, Chemistry Commons, Molecular Biology Commons
